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Justin Gatlin finished a 100m race limping and holding his forearm just three weeks before the scheduled defence of his world title in Doha.
The 37-year-old American left the track supported by a fellow athlete after finishing fourth in 10.29 seconds in the Croatian capital Zagreb.
Compatriot Michael Rodgers won the race in the IAAF World Challenge assembly at a wind-assisted 10.04.
Gatlin (9.87) gets the fourth fastest time in the world this year.
Fellow American Christian Coleman has been fastest in 2019 with 9.81 minutes and is free to run in Doha after possible charges for lost three drugs tests have been dropped.
Coleman was second in the 2017 World Championships behind Gatlin, the 2004 Olympic 100m champion who served that a ban between 2006 and 2010.
In Croatia, Gatlin – who is expecting to compete second year at Tokyo – abruptly winced in pain at approximately the mark.
Lady Erika Rosenbaum explains her encounter
It has among the broadest ranges of choice in sport, from jumps and cries to space and cross country running or sprints.
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